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smrna-fish healthy and scc human skin tissues nbp2-30229  (Novus Biologicals)


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    Novus Biologicals smrna-fish healthy and scc human skin tissues nbp2-30229
    Smrna Fish Healthy And Scc Human Skin Tissues Nbp2 30229, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smrna-fish healthy and scc human skin tissues nbp2-30229/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    smrna-fish healthy and scc human skin tissues nbp2-30229 - by Bioz Stars, 2026-04
    90/100 stars

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    (A) Human skin squamous cell cancer tissue array was immunostained with anti-MET antibody and visualized by bright-field microscopy. (B) Quantification of the epithelial compartment staining intensity using the Aperio software ImageScope according to tumor grade. Grade1 (n=49), grade 2 (n=20) grade 3 (n=5). *P < 0.05 vs. grade1. (C) RNA in situ and protein IHC in human squamous cell carcinomas. RNA in situ signal (red) deconvoluted with Aperio ScanScope algorithm and pseudo-colored with Adobe imaging software. Bar = 200 μm. Both positive and negative tumors are shown for ISH and IHC. (D) Human SCC cell lines were grown to confluence and treated for 24h with DMSO, Capmatinib (Cap.), PHA665752 (PHA) or recombinant HGF. Total cell extracts were analyzed by immunoblotting for phospho-MET, total MET and HSP 90 (D) and in separate vessels tritiated-thymidine incorporation was measured (E). *P < 0.05, **P < 0.01, ***P < 0.00,1 ***P < 0.001, ****P < 0.0001 vs control (0). Bars represent the mean ± SEM value of quadruplicates.

    Journal: Science signaling

    Article Title: MET signaling in keratinocytes activates EGFR and initiates squamous carcinogenesis

    doi: 10.1126/scisignal.aaf5106

    Figure Lengend Snippet: (A) Human skin squamous cell cancer tissue array was immunostained with anti-MET antibody and visualized by bright-field microscopy. (B) Quantification of the epithelial compartment staining intensity using the Aperio software ImageScope according to tumor grade. Grade1 (n=49), grade 2 (n=20) grade 3 (n=5). *P < 0.05 vs. grade1. (C) RNA in situ and protein IHC in human squamous cell carcinomas. RNA in situ signal (red) deconvoluted with Aperio ScanScope algorithm and pseudo-colored with Adobe imaging software. Bar = 200 μm. Both positive and negative tumors are shown for ISH and IHC. (D) Human SCC cell lines were grown to confluence and treated for 24h with DMSO, Capmatinib (Cap.), PHA665752 (PHA) or recombinant HGF. Total cell extracts were analyzed by immunoblotting for phospho-MET, total MET and HSP 90 (D) and in separate vessels tritiated-thymidine incorporation was measured (E). *P < 0.05, **P < 0.01, ***P < 0.00,1 ***P < 0.001, ****P < 0.0001 vs control (0). Bars represent the mean ± SEM value of quadruplicates.

    Article Snippet: To address the frequency of MET expression in human cutaneous SCC, we used human skin SCC tissue arrays (BIOmax) to probe for MET by immunohistochemical staining and histomorphometric analysis using the Aperio software.

    Techniques: Microscopy, Staining, Software, In Situ, Imaging, Recombinant, Western Blot, Control